Rat VIP (Vasoactive Intestinal Peptide) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1454MS-48, ELK1454MS-96, ELK1454MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Rat
Alternative Names: IL-40; UNQ464
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-40 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q6UX52
Research Use Only