Recombinant 2019-nCoV NSP8 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSR030470-10, PKSR030470-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NSP8
Target Synonym: SARS-CoV 2 nsp8
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: C-6His
Accession: YP_009725304.1
Background: Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.
Sequence: Ala1-Gln198
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 25 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only