Recombinant HIF1 beta Monoclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: E-AB-81565-50, E-AB-81565-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: HIF1 beta
Target Synonym: arnT; ARNT protein; ARNT; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF-1-beta; HIF1-beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Hypoxia-inducible factor 1-beta; nuclear translocator; Tango
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Hamster
Application: WB, IHC-P
Isotype: IgG
Clonality: Rabbit Monoclonal
Clone No.: R05-3B2
UNIProt ID: P27540
Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).
Concentration: 300 μg/mL
Immunogen: Recombinant protein of human HIF1 beta
Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method: Affinity Purified
Dilution: WB 1:500-1:1000 IHC 1:50-1:100
Calculated MW: 87kDa
Observed MW: 87kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only