Recombinant HMGB1 Monoclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: E-AB-81436-50, E-AB-81436-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: HMGB1
Target Synonym: Amphoterin; Chromosomal protein; nonhistone; HMG1; DKFZp686A04236; High mobility group 1; High mobility group box 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG-1; HMG1; HMG3; HMGB 1; HMGB1; HMGB1; NONHISTONE CHROMOSOMAL PROTEIN HMG1; SBP 1; Sulfoglucuronyl carbohydrate binding protein
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC-P
Isotype: IgG
Clonality: Rabbit Monoclonal
Clone No.: R07-2D8
UNIProt ID: P09429
Background: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
Concentration: 300 μg/mL
Immunogen: A synthetic peptide of human HMGB1
Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method: Affinity Purified
Dilution: WB 1:1000-1:2000 IHC 1:50-1:100
Calculated MW: 25kDa
Observed MW: 25kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only