Recombinant Human β-Actin/Beta Actin Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033261-10, PKSH033261-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: β-Actin; Beta Actin
Target Symptom: Actin Cytoplasmic 1; Beta-Actin; ACTB
Research Area: Signal Transduction; Tags & Cell Markers; Isotypeloading control;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
UNIProt ID: P60709
Accession: P60709
Background: Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Sequence: Asp2-Phe375
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl, 0.1% TritonX-100, 2mM DTT, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 42.8 kDa
Observed MW: 30-100 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only