Recombinant Human β-NGF/NGFB Protein (aa 122-241, E. coli)(Active)
Size: 100μg
Catalogue Number: PKSH033269-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: β-NGF; NGFB
Target Symptom: Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB
Research Area: Neuroscience
Target Species: Human
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
UNIProt ID: P01138
Accession: P01138
Background: Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.
Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.3 ng/ml.
Sequence: Ser122-Ala241
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 13.4 kDa
Observed MW: 14 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only