Product Description

Recombinant Human ACE-2 (C-6His)

Size: 50µg

Catalogue Number: EPT135-50

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

Description: Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a 6His tag at the C-terminus.

Alternate Names: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-Converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, ACE2

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Immobilized Human ACE-2-His (Cat#C419) at 2μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-Fc (Cat#DRA121) . The ED50 of 2019-nCoV S Protein RBD (N501Y, C-Fc) (Cat#DRA121) is 3.57 ng/ml.

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Molecular Weight: 84.63 KDa

Observed Molecular Weight: 103 KDa, reducing conditions

Storage: Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Accession: Q9BYF1

Research Use Only