Product Description

Recombinant Human/Cynomolgus CD28 (C-Fc)

Size: 10µg

Catalogue Number: EPT281-10

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: T-cell-specific surface glycoprotein CD28 (CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin (Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Description: Recombinant Human/Cynomolgus T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a Fc tag at the C-terminus.

Alternate Names: CD28, CD28 antigen, CD28 molecule, T-cell-specific surface glycoprotein CD28, Tp44, TP44

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Immobilized Anti-CD28 mAb (Cat#GMP-A063) at 2μg/ml (100 μl/well) can bind Human/Cynomolgus CD28-Fc * (Cat#CX81) *: Biotinylated by NHS-biotin prior to testing The ED50 of Human/Cynomolgus CD28-Fc * (Cat#CX81) is 83.5 ng/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Molecular Weight: 42.3 KDa

Observed Molecular Weight: 60 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P10747

Research Use Only