Recombinant Human Galectin 9 (C-6His) - EPT189

Recombinant Human Galectin 9 (C-6His)

Size: 50ug

Catalogue Number: EPT189-50

Citations, Manuals and MSDS Available upon request.

Background: Galectin-9 is a cytoplasmic protein that contains two galectin domains. Galectin-9 is an S-type lectin that is over-expressed in Hodgkin's disease tissue. Galectin-9 binds galactosides and has high affinity for the Forssman pentasaccharide. Galectin-9 plays a role in thymocyte-epithelial interactions relevant to the biology of the thymus and Inhibits cell proliferation. Galectin-9 is a ligand for HAVCR2/TIM3 and induces T-helper type 1 lymphocyte (Th1) death. In addition, Galectin-9 suppresses tumor cell metastasis by interfering with the associations CD44, VCAM-1, Integrin α4β1.

Alternate Names: Galectin-9; Gal-9; Ecalectin; Tumor Antigen HOM-HD-21; LGALS9

Description: Recombinant Human Galectin 9 is produced by our Mammalian expression system and the target gene encoding Met1-Thr323 is expressed with a 6His tag at the C-terminus.

Accession: Q3B8N1-2

Molecular Weight: 36.7 KDa

Apparent Molecular Weight: 49 KDa, reducing conditions

Storage Condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Biological Activity: Immobilized Human Galectin 9-His (Cat#C808) at 10μg/ml (100 μl/well) can bind Human TIM-3-His* (Cat#C356).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human TIM-3-His (Cat#C356) is 8-40 ug/ml.

Redissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Research Use Only