Recombinant Human IFN-λ3/IL-28B Protein (Human Cells, His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032637-10, PKSH032637-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: IFN-λ3; IL-28B
Target Symptom: Interleukin-28B; IL-28B; Cytokine Zcyto22; Interferon Lambda-3; IFN-Lambda-3; Interferon Lambda-4; IFN-Lambda-4; Interleukin-28C; IL-28C; IL28B; IFNL3; IFNL4; IL28C; ZCYTO22
Research Area: Signal Transduction; Microbiology; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: Q8IZI9
Accession: Q8IZI9
Background: Interleukin-28B; also known as Cytokine Zcyto22; Interferon lambda-3; Interferon lambda-4; IFNL3; IFNL4; ZCYTO22 and IL28B; is a secreted cytokine which belongs to the IL-28/IL-29 family. IL-28 has also been shown to play a role in the adaptive immune response. IL28B has immunomodulatory activity and up-regulates MHC class I antigen expression. IL28B displays potent antiviral activity and antitumor activity. In addition; IL28B is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Activity: Immobilized Human IL-28-His at 10μg/ml(100 μl/well) can bind Human IL-10RB-Fc(Cat: PKSH032564). The ED50 of Human IL-28-His is 0.37 ug/ml.
Sequence: Val22-Val196
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; 1mM EDTA; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.7 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only