Product Description

Recombinant Human IFN gamma (E. coli)

Size: 50µg

Catalogue Number: EPT184-50

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.

Description: Recombinant Human Interferon Gamma is produced by our E. coli expression system and the target gene encoding Gln24-Gln166 is expressed.

Alternate Names: Interferon Gamma, IFN-Gamma, Immune Interferon, IFNG

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

Molecular Weight: 16.88 KDa

Observed Molecular Weight: 16 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P01579

Research Use Only