Product Description

Recombinant Human IL-3 (N-6His)

Size: 10µg

Catalogue Number: EPT177-10

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity, and is thought to be associated with neurologic disorders.

Description: Recombinant Human Interleukin-3 is produced by our E. coli expression system and the target gene encoding Ala20-Phe152 is expressed with a 6His tag at the N-terminus.

Alternate Names: Interleukin-3, IL-3, Hematopoietic Growth Factor, Mast Cell Growth Factor, MCGF, Multipotential Colony-Stimulating Factor, P-Cell-Stimulating Factor, IL3

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Loaded Human IL-3RA-Fc (Cat#CU77) on Protein A Biosensor, can bind Human IL-3 (Cat#CF63) with an affinity constant of 3.89uM as determined in BLI assay. 

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Molecular Weight: 16.6 KDa

Observed Molecular Weight: 13-16 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P08700

Research Use Only