Recombinant Human LDLR Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033435-10, PKSH033435-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: LDLR
Target Synonym: Low-Density Lipoprotein Receptor; LDL Receptor; LDLR; FH; FHC; LDL R; LDL Receptor; LDLCQ2
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: NP_000518.1
Background: Low-Density Lipoprotein Receptor (LDLR) is a transmembrane glycoprotein that plays a critical role in cholesterol homeostasis. LDLR mediates blood cholesterol level by interacting with lipoprotein particles like LDL and VLDL. The extracellular domain of LDLR contains LDL receptor type A (ligand-binding) modules (LA repeats), epidermal growth factor-like modules, and LY repeats containing the YWTD consensus motif that are important in binding and releasing of ApoB-100 and ApoE in lipoprotein particles. The C terminal domain of LDLR inside the cell is required for the receptor internalization. Loss of function mutations in the LDLR gene causes Familial Hypercholesterolemia (FH).
Sequence: Ala22-Arg788
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 84.8 kDa
Observed MW: 99-135 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only