Product Description

Recombinant Human/Murine/Rat BDNF

Size: 50µg

Catalogue Number: EPT036-50

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75) . BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.

Description: Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E. coli expression system and the target gene encoding His129-Arg247 is expressed.

Alternate Names: Brain-Derived Neurotrophic Factor, BDNF, Abrineurin

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Immobilized Human TrkB-His (Cat#C507) at 2ug/ml (100 μl/well) can bind Human BDNF* (Cat#C076) *: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF* (Cat#C076)  is 47.58 ng/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

Molecular Weight: 13 KDa

Observed Molecular Weight: 14 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P23560

Research Use Only