Recombinant human Olfactomedin-4/OLFM4 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01761-10, RP01761-20, RP01761-50, RP01761-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant human Olfactomedin-4/OLFM4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp21-Gln510) of human Olfactomedin-4/OLFM4 (Accession #NP_006409.3) fused with a 6×His tag at the C-terminus.
Alternate Names: GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
SWISS: Q6UX06
GeneID: 10562
Species: human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein. It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers , and is up-regulated in epithelial cells during inflammation. Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions. Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells. OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer.
Source: HEK293 cells
Tag: C-6His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: DLGDVGPPIPSPGFSSFPGVDSSSSFSSSSRSGSSSSRSLGSGGSVSQLFSNFTGSVDDRGTCQCSVSLPDTTFPVDRVERLEFTAHVLSQKFEKELSKVREYVQLISVYEKKLLNLTVRIDIMEKDTISYTELDFELIKVEVKEMEKLVIQLKESFGGSSEIVDQLEVEIRNMTLLVEKLETLDKNNVLAIRREIVALKTKLKECEASKDQNTPVVHPPPTPGSCGHGGVVNISKPSVVQLNWRGFSYLYGAWGRDYSPQHPNKGLYWVAPLNTDGRLLEYYRLYNTLDDLLLYINARELRITYGQGSGTAVYNNNMYVNMYNTGNIARVNLTTNTIAVTQTLPNAAYNNRFSYANVAWQDIDFAVDENGLWVIYSTEASTGNMVISKLNDTTLQVLNTWYTKQYKPSASNAFMVCGVLYATRTMNTRTEEIFYYYDTNTGKEGKLDIVMHKMQEKVQSINYNPFDQKLYVYNDGYLLNYDLSVLQKPQ
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only