Recombinant Human SIRP-beta 1/CD172b Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01351-10, RP01351-20, RP01351-50, RP01351-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human SIRP-beta 1/CD172b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu30-Leu371) of human SIRPB1/CD172b (Accession #NP_006056.2) fused with a 6×His tag at the C-terminus.
Alternate Names: 9930027N05Rik, SIRP-beta, Sirpb, Sirpb1, SIRPB1
SWISS: O00241
GeneID: 10326
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Signal-regulatory protein beta-1 (SIRP beta 1) also known as SIRP1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily.Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. SIRP are classified as SIRP alpha molecules, containing 11- to 113-amino acid long, or SIRP beta molecules, with a 5-amino acid long intracytoplasmic domain. SIRP beta 1 is a new DAP12-associated receptor involved in the activation of myeloid cells, which contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. SIRP beta 1 acts as an activating isoform of SIRP alpha molecules, confirming the co-existence of inhibitory ITIM-bearing molecules, recruiting SHP-1 and SHP-2 protein tyrosine phosphatases, and activating counterparts, whose engagement couples to protein tyrosine kinases via ITAM-bearing molecules.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPL
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only