Recombinant Human Thymidylate Kinase/DTYMK Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033115-10, PKSH033115-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Thymidylate Kinase; DTYMK
Target Synonym: Thymidylate kinase; dTMP kinase; DTYMK; CDC8; TMPK; TYMK
UNIProt ID: P23919
Research Areas: epigenetics and nuclear signal
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P23919
Background: Thymidylate kinase (DTYMK) is a ubiquitous enzyme of about 25 kD which belongs to thymidylate kinase family. DTYMK is important in the dTTP synthesis pathway for DNA synthesis. It participated in the pyrimidine metabolism pathway and dTTP biosynthesis pathway. DTYMK catalyzes the conversion of dTMP to dTDP and catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP) in the presence of ATP and magnesium. Structural and functional analyses suggest that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.
Sequence: Met 1-Lys212
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 24.9 kDa
Observed MW: 20 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only