Recombinant Monoclonal Antibody to Human MANF
Size: 100 µg
Catalogue Number: 341-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human MANF
Immunogen Description: Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
UniProt ID: P55145
Alternative Names: ARMET, ARP
Clonality: Recombinant monoclonal antibody
Clone: 2B8, chicken-mouse IgG2a chimeric antibody
Class: mouse IgG2a
Reactivity: Human MANF
Application: ELISA, IF, IHC-C
Protocol: ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml.
Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: MabSelect affinity chromatography
Buffer: PBS pH 7.4, with 0.1% sodium azide
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at –20 to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Research Use Only