Product Description

Recombinant Mouse Beta-NGF (110AA)

Size: 10µg

Catalogue Number: EPT250-10

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF) , neurotrophin-3 (NT-3) , and neurotrophin-4 (NT-4) . These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Description: Recombinant Mouse Beta-Nerve Growth Factor is produced by our E. coli expression system and the target gene encoding Met130-Arg239 is expressed.

Alternate Names: Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB,β-NGF

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 68.52 ng/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Molecular Weight: 12.4 KDa

Observed Molecular Weight: 12 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P01139

Research Use Only