Product Description

Recombinant Mouse IL-10

Size: 10µg

Catalogue Number: EPT173-10

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF) , IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) .Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81% identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.

Description: Recombinant Mouse Interleukin-10 is produced by our E. coli expression system and the target gene encoding Ser19-Ser178 is expressed.

Alternate Names: Interleukin-10, Il10, IL-10, Cytokine synthesis inhibitory factor, CSIF

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Measured in a cell proliferation assay using FDC-P1 Mouse bone marrow cells The ED50 for this effect is 6 ng/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

Molecular Weight: 18.9 KDa

Observed Molecular Weight: 16 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P18893

Research Use Only