Product Description

Recombinant Mouse IL-4

Size: 10µg

Catalogue Number: EPT035-10

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mouse Interleukin-4 (IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α­helix structure. IL­4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL­4 is primarily expressed by Th2­biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL­4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Description: Recombinant Mouse Interleukin-4 is produced by our E. coli expression system and the target gene encoding His23-Ser140 is expressed.

Alternate Names: Interleukin-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1, IL-4, BSF-1

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells.  The ED50 for this effect is 0.01 ng/ml.

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

Molecular Weight: 13.4 KDa

Observed Molecular Weight: 14 KDa, reducing conditions

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Accession: P07750

Research Use Only