Recombinant Vimentin Monoclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: E-AB-81438-50, E-AB-81438-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Vimentin
Target Synonym: CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin
Research Areas: Cancer, Developmental Biology, Neuroscience, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Hamster
Application: WB, IHC-P, IF
Isotype: IgG
Clonality: Rabbit Monoclonal
Clone No.: R02-8C4
UNIProt ID: P08670
Background: Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Concentration: 300 μg/mL
Immunogen: A synthetic peptide of human Vimentin
Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method: Affinity Purified
Dilution: WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Calculated MW: 54kDa
Observed MW: 54kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only