Product Description

Repac Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8099-50, ES8099-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Members of the RAS (see HRAS, MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: RAPGEF5, GFR, KIAA0277, MRGEF, Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, MR-GEF, Related to Epac, Repac

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RAPGEF5. AA range:431-480

Isotype: IgG

Target: Repac

Specificity: Repac Polyclonal Antibody detects endogenous levels of Repac protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed with highest levels in brain,

Observed Band (KD): 60kD

Fields: Ras signaling pathway, Rap1 signaling pathway

Function: Function: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation
Similarity: Contains 1 N-terminal Ras-GEF domain
Similarity: Contains 1 Ras-GEF domain
Tissue Specificity: Widely expressed with highest levels in brain

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: RAPGEF5

Protein Name: Rap guanine nucleotide exchange factor 5

Mouse Gene ID: 217944

Mouse SwissProt Number: Q8C0Q9

Rat Gene ID: 362799

Rat SwissProt Number: P83900

Research Use Only