Product Description

Rho B Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6040-50, ES6040-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: function:Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.,miscellaneous:RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.,PTM:Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.,similarity:Belongs to the small GTPase superfamily. Rho family.,subcellular location:Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.,subunit:Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.,

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: RHOB, ARH6, ARHB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RHOB. AA range:99-148

Isotype: IgG

Target: Rho B

Specificity: Rho B Polyclonal Antibody detects endogenous levels of Rho B protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Aorta, Brain, Fetal brain cortex, Hippocampus,

Observed Band (KD): 22kD

Fields: Salmonella infection

Function: Function: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development
Miscellaneous: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects
PTM: Prenylation specifies the subcellular location of

Subcellular Location: Late endosome membrane, Lipid-anchor Cell membrane, Lipid-anchor Nucleus Cleavage furrow Late endosomal membrane (geranylgeranylated form) Plasma membrane (farnesylated form) Also detected at the nuclear margin and in the nucleus Translocates to the equatorial region before furrow formation in a ECT2-dependent manner

Storage and Stability: -20°C/1 year

Gene Name: RHOB

Protein Name: Rho-related GTP-binding protein RhoB

Mouse Gene ID: 11852

Mouse SwissProt Number: P62746

Rat Gene ID: 64373

Rat SwissProt Number: P62747

Research Use Only