Product Description

RIN1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8073-50, ES8073-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: function:Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.,PTM:Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylated on Ser-351 by PKD.,similarity:Belongs to the RIN (Ras interaction/interference) family.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 VPS9 domain.,subcellular location:Some amount is membrane-associated.,subunit:Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.,tissue specificity:Expressed in all tissues examined with high levels in brain, placenta and pancreas.,

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: RIN1, Ras and Rab interactor 1, Ras inhibitor JC99, Ras interaction/interference protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RIN1. AA range:655-704

Isotype: IgG

Target: RIN1

Specificity: RIN1 Polyclonal Antibody detects endogenous levels of RIN1 protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in all tissues examined with high levels in brain, placenta and pancreas,

Observed Band (KD): 84kD

Fields: Ras signaling pathway

Function: Function: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis
PTM: Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylated on Ser-351 by PKD
Similarity: Belongs to the RIN (Ras interaction/interference) family
Similarity: Contains 1 Ras-associating domain
Similarity: Contains 1 SH2 domain
Similarity: Contains 1 VPS9 domain
Subcellular Location: Some amount is membrane-associated
Subunit: Interacts with the GTP-boun

Subcellular Location: Cytoplasm Membrane Cytoplasm, cytoskeleton Some amount is membrane-associated

Storage and Stability: -20°C/1 year

Gene Name: RIN1

Protein Name: Ras and Rab interactor 1

Mouse Gene ID: 225870

Mouse SwissProt Number: Q921Q7

Rat Gene ID: 207119

Rat SwissProt Number: P97680

Research Use Only