Product Description

Rit1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3383-50, ES3383-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: RIT1, RIBB, RIT, ROC1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RIT1. AA range:170-219

Isotype: IgG

Target: Rit1

Specificity: Rit1 Polyclonal Antibody detects endogenous levels of Rit1 protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in many tissues,

Observed Band (KD): 25kD

Function: Enzyme Regulation: Alternate between an inactive form bound to GDP and an active form bound to GTP
Function: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation
Miscellaneous: Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members
Miscellaneous: Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation
Similarity: Belongs to the small GTPase superfamily. Ras family
Subunit: Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state. Interacts with RGL3
tissue specificit

Subcellular Location: Cell membrane

Storage and Stability: -20°C/1 year

Gene Name: RIT1

Protein Name: GTP-binding protein Rit1

Mouse Gene ID: 19769

Mouse SwissProt Number: P70426

Research Use Only