Product Description

RORγ Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: GCP109-50, GCP109-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51449

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:1000~1:2000
IHC: 1:50~1:200

Background: The nuclear orphan receptors RORα and RORg are members of the nuclear hormone receptor superfamily. Members of this family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORα and RORg are unique in that they bind DNA as monomers. RORα has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORg comprises a 560 amino acid protein that shares 50% amino acid identity with RORα and is most highly expressed in skeletal muscle. Although these proteins are considered “orphan receptors,” due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. The gene encoding RORα maps to chromosome 15q22.2 and the gene encoding RORg maps to chromosome 1q21.3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RORγ polyclonal antibody detects endogenous levels of RORγ protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RORγ polyclonal antibody at 1:1000 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Human Brain using RORγ antibody at dilution of 1:50., Western blot (WB) analysis of RORγ polyclonal antibody at 1:1000 dilution
Lane 1: RORγ knockdown A549 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)

Alternative Name: Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; RORC; NR1F3; RORG; RZRG

Immunogen: Synthetic peptide, corresponding to Human RORγ.

Conjugate: Unconjugated

Modification: Unmodified