Product Description

RPA70 Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS49499-50, BS49499-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellμlar response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that woμld interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regμlating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, ICC/IF, FC, IP

Alternate Names: HSSB, RF-A, RP-A, REPA1, RPA70, MST075

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human RPA70

Dilution: WB: 1:1000-1:5000 IHC: 1:20-1:100 ICC/IF: 1:20-1:50 FC: 1:20 IP: 1:20

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27694

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only