rT3 (Reverse Triiodothyronine) ELISA Kit - ELK7898

rT3 (Reverse Triiodothyronine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7898-48, ELK7898-96, ELK7898-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4- (4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

Assay Type: Competitive Inhibition

Sensitivity: 27.68 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Infection immunity; Endocrinology; Hormone metabolism; Autoimmunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only