Product Description

rT3 (Reverse Triiodothyronine) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7898-48, ELK7898-96, ELK7898-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: RT3, r-T3, Reverse T3, (2S)-2-Amino-3-[4-(4-Hydroxy-3, 5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid, 3, 3', 5'-Triiodo-L-thyronine

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Infection Immunity, Endocrinology, Hormone Metabolism, Autoimmunity

Assay Length: 2h

Sensitivity: 27.68 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Research Use Only