Product Description

RUFY1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7614-50, ES7614-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: RUN and FYVE domain containing 1(RUFY1) Homo sapiens This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: RUFY1, RABIP4, ZFYVE12, RUN and FYVE domain-containing protein 1, FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, Zinc finger FYVE domain-containing protein 12

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RUFY1. AA range:139-188

Isotype: IgG

Target: RUFY1

Specificity: RUFY1 Polyclonal Antibody detects endogenous levels of RUFY1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Broadly expressed, with highest levels in lung, testis, kidney and brain,

Observed Band (KD): 80kD

Fields: Endocytosis

Function: Domain: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate
Function: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
PTM: Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting
Similarity: Contains 1 FYVE-type zinc finger
Similarity: Contains 1 RUN domain
Subunit: Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding
Tissue Specificity: Broadly expressed, with highest levels in lung, testis, kidney and brain

Subcellular Location: Cytoplasm Early endosome membrane, Peripheral membrane protein

Storage and Stability: -20°C/1 year

Gene Name: RUFY1

Protein Name: RUN and FYVE domain-containing protein 1

Mouse Gene ID: 216724

Mouse SwissProt Number: Q8BIJ7

Research Use Only