RVT (Resveratrol) ELISA Kit - ELK8265

RVT (Resveratrol) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8265-48, ELK8265-96, ELK8265-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: trans-3,5,4'-Trihydroxystilbene; 3,4',5-Stilbenetriol; trans-Resveratrol

Assay Type: Competitive Inhibition

Sensitivity: 91.1 ng/mL

Standard: 20000 ng/mL

Detection Range: 312.5-20000 ng/mL

Sample Type: plant tissue and biological fluids

Assay Length: 2h

Research Areas: Cardiocerebral vascular system

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with RVT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RVT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RVT in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only