S1P (Sphingosine-1-Phosphate) ELISA Kit - ELK8273

S1P (Sphingosine-1-Phosphate) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8273-48, ELK8273-96, ELK8273-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Sphingosine-1-Phosphate

Assay Type: Competitive Inhibition

Sensitivity: 4.48 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Ovarian dysfunction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S1P. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only