Sarcoglycan-α Polyclonal Antibody-BS5920

Sarcoglycan-α Polyclonal Antibody

Catalogue Numbers: BS5920-50, BS5920-100

Sizes: 50µl, 100µl

Alternative Name: Alpha-sarcoglycan; Sarcoglycan α; α-Sarcoglycan; α-SG; SG-α; SGα; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2; Dystroglycan 2; Dystroglycan2; SGCA; ADL; DAG2;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16586

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC IP

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200
IP: 1:50~1:200

Background: The sarcoglycan transmembrane proteins are members of the dystrophin complex. Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle fibers. Four sarcoglycan subunit proteins, designated α-, β-, γ- and δ-sarcoglycan, form a complex on the skeletal muscle cell surface membrane. A genetic defect in any one of these proteins causes the loss or marked decrease of the whole sarcoglycan complex, which is observed in the autosomal recessive muscular dystrophy, sarcoglycanopathy. In smooth muscle, β- and δ-sarcoglycans are associated with ε-sarcoglycan, a glycoprotein homologous to α-sarcoglycan. Additionally, a complete deficiency in δ-sarcoglycan is the cause of the Syrian hamster BIO.14 cardiomyopathy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Sarcoglycan-α polyclonal antibody detects endogenous levels of Sarcoglycan-α protein.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Sarcoglycan-α pAb at 1:500 dilution
Lane 1: AML-12 whole cell lysate(40ug)
Lane 2: H9C2 whole cell lysate(40ug)
Lane 3: A549 whole cell lysate(40ug)
Lane 4: EC9706 whole cell lysate(40ug)
Lane 5: H1792 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 209-254 of Human Sarcoglycan-α.

Conjugate: Unconjugated

Modification: Unmodified