Product Description

SARDH Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES13199-50, ES13199-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human SARDH AA range: 279-329

Isotype: IgG

Target: SARDH

Specificity: This antibody detects endogenous levels of SARDH at Human/Mouse/Rat

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in pancreas, liver and kidney,

Molecular Weight (Da): 101kD

Fields: Glycine, serine and threonine metabolism, Metabolic pathways

Function: Catalytic Activity: Sarcosine + acceptor + H(2)O = glycine + formaldehyde + reduced acceptor
Cofactor: Binds 1 FAD covalently per monomer
Disease: Defects in SARDH are a ause of sarcosinemia [MIM: 268900]. Sarcosinemia is a metabolic disorder characterized by an increased concentration of sarcosine in plasma and urine due to sarcosine dehydrogenase deficiency. Prevalence has been estimated at 1: 28'000 to 1: 350'000 in newborn screening programs. Sarcosinemia is most probably a benign condition without significant clinical problems. It is transmitted in an autosomal recessive manner
Pathway: Amine and polyamine degradation, sarcosine degradation, formaldehyde and glycine from sarcosine: step 1/1
Similarity: Belongs to the gcvT family

Subcellular Location: Mitochondrion matrix

Storage and Stability: -20°C/1 year

Gene Name: SARDH DMGDHL1

Protein Name: SARDH

Mouse Gene ID: 192166

Mouse SwissProt Number: Q99LB7

Rat Gene ID: 114123

Rat SwissProt Number: Q64380

Research Use Only