Product Description

Separase Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3423-50, ES3423-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21, MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: ESPL1, ESP1, KIAA0165, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816

Isotype: IgG

Target: Separase

Specificity: Separase Polyclonal Antibody detects endogenous levels of Separase protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Bone marrow, Epithelium,

Observed Band (KD): 230kD

Fields: Cell cycle, Oocyte meiosis, Human T-cell leukemia virus 1 infection

Function: Catalytic Activity: All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage
Enzyme Regulation: Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1126 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin

Subcellular Location: Cytoplasm Nucleus

Storage and Stability: -20°C/1 year

Gene Name: ESPL1

Protein Name: Separin

Mouse Gene ID: 105988

Mouse SwissProt Number: P60330

Research Use Only