Product Description

Shank 2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5277-50, ES5277-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC

Alternate Names: SHANK2, CORTBP1, KIAA1022, SH3 and multiple ankyrin repeat domains protein 2, Shank2, Cortactin-binding protein 1, CortBP1, Proline-rich synapse-associated protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human SHANK2. AA range:331-380

Isotype: IgG

Target: Shank 2

Specificity: Shank 2 Polyclonal Antibody detects endogenous levels of Shank 2 protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform 3 is present in epithelial colonic cells (at protein level),

Observed Band (KD): 135kD

Fields: Glutamatergic synapse

Function: Alternative Products: Additional isoforms seem to exist,Domain: The PDZ domain is required for interaction with GRID2, PLCB3, CFTR and SLC9A3
Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction
Similarity: Belongs to the SHANK family
Similarity: Contains 1 PDZ (DHR) domain
Similarity: Contains 1 SAM (sterile alpha motif) domain
Subcellular Location: Cytoplasm, postsynaptic density of neuronal cells
Subunit: Interacts with CCTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with GRID2, SLC9A3, CFTR and PLCB3.

Subcellular Location: Apical cell membrane Cytoplasm Cell junction, synapse Cell junction, synapse, postsynaptic density Cell projection, growth cone Cell projection, dendritic spine Colocalizes with cortactin in growth cones in differentiating hippocampal neurons Colocalized with PDE4D to the apical membrane of colonic crypt cells (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: SHANK2

Protein Name: SH3 and multiple ankyrin repeat domains protein 2

Mouse SwissProt Number: Q80Z38

Research Use Only