Product Description

Sheep CRH (Corticotropin Releasing Hormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11860-48, ELK11860-96, ELK11860-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Sheep

Alternate Names: CRF, Corticotropin Releasing Factor, Corticoliberin

Sensitivity: 0.11 ng/mL

Assay Type: Competitive Inhibition

Standard: 20 ng/mL

Detection Range: 0.312-20 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 2h

Research Area: Endocrinology, Cardiovascular Biology, Neuro Science, Hormone Metabolism, Urology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P01142

Research Use Only