Sheep GHRL (Ghrelin) ELISA Kit - ELK7969

Sheep GHRL (Ghrelin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7969-48, ELK7969-96, ELK7969-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: MTLRP; Growth Hormone-Releasing Peptide

Assay Type: Competitive Inhibition

Sensitivity: 49.7 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, cell lysates, saliva, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only