Sheep STAT5B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit - ELK9832

Sheep STAT5B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9832-48, ELK9832-96, ELK9832-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: STAT5-B

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep STAT5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep STAT5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep STAT5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep STAT5B in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only