Sheep T3 (Triiodothyronine) ELISA Kit - ELK0550

Sheep T3 (Triiodothyronine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0550-48, ELK0550-96, ELK0550-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Assay Type: Competitive Inhibition

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2.5h

Research Areas: Endocrinology; Autoimmunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only