Product Description

Sheep T4 (Thyroxine) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0549-48, ELK0549-96, ELK0549-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 3, 5, 3', 5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine

Reactivity: Sheep

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Endocrinology, Hormone Metabolism

Assay Length: 2.5h

Sensitivity: 1.83 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Research Use Only