Sheep vWF (Von Willebrand Factor) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10102-48, ELK10102-96, ELK10102-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: F8VWF; VWD; von Willebrand antigen 2
Assay Type: Sandwich
Sensitivity: 0.049 µg/mL
Standard: 10 µg/mL
Detection Range: 0.16-10 µg/mL
Sample Type: Plasma
Assay Length: 3.5h
Research Areas: Signal transduction; Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep vWF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only