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si3D-FectIN™ 3D Transfection Reagent
si3D-FectIN™ 3D Transfection Reagent
si3D-FectIN™ 3D Transfection Reagent

si3D-FectIN™ 3D Transfection Reagent

Regular price
$142.00 USD
Regular price
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$142.00 USD
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si3D-FectIN™ is a 3D transfection reagent specifically designed and developed for silencing gene expression in cells cultured in gels (or hydrogels).

3D matrices not only add a third dimension to the cells’ environment, they also allow creating significant differences in cellular phenotype and behaviour. In this way, 3D matrices bearing complexes formed with si3D-FectIN™ reagent and siRNA are colonized by cells to be transfected in a more natural environment. 


For DNA Transfection, please refer to 3D-FectIN

  • Highly efficient for gene silencing in 3D matrices
  • Ideal for any gel and hydrogel
  • Dedicated to short nucleic acid sequences (siRNA, miRNA…)
  • Long term gene silencing
  • Universal (primary cells and cell lines)
  • Serum Compatible
 

Sizes:

  • 250 µL (STN50250): Good for 30-65 transfections with 50 nM siRNA in 50 µL Gel
  • 500 µL (STN50500): Good for 65-125 transfections with 50 nM siRNA in 50 µL Gel
  • 1 mL (STN51000): Good for 125-250 transfections with 50 nM siRNA in 50 µL Gel
 

Storage: +4°C

Shipping Conditions: Room temperature

 

Application

Perfect for all transfection applications in 3D hydrogels such as collagen, hyaluronic acid, PEG, fibrin, laminin:

  • angiogenesis
  • tube and acini formation
  • colonization
  • neurite growth
  • tissue engineering
  • tissue regeneration
  • tumour invasion
  • neural differentiation
  • cellular polarization
  • tissue formation…


RECOMMENDED FOR: Gene silencing of cells growing in 3D-hydrogels.

 

Results

siRNA Transfection in hydrogels with si3D-FectIN Reagent

Figure 1: si3D-FectIN™ allows efficient siRNA transfection in hydrogels: Collagen-derived hydrogels were loaded with 50 nM of fluorescently labelled siRNA complexed to 4 µL of si3D-FectIN™ transfection reagent. Transfection efficiency of COS-7 and NIH-3T3 cells was observed after 48 h by fluorescence microscopy.

 

gene silencing in 3D-hydrogels with si-3D-FectIN Reagent

Figure 2: si3D-FectIN™allows optimal gene silencing in 3D-hydrogels: Collagen-derivedhydrogels were loaded with complexes formed by 4 µL si3D-FectIN™ and several concentrations of siRNA (5-50mM) directed against GFP. NIH-3T3-GFP were added and gene silencing was observed after 72 h by fluorescence microscopy.