3D-FectIN™ is the newest 3D-Transfection Reagent specifically developed to directly transfect cells cultured in 3D hydrogels. 3D-FectIN™ is suitable for all kind of hydrogels and cells. 3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behaviours.
Because 3D matrices are routinely used in basic research and therapeutic applications, OZ Biosciences has developed 3D-FectIN™ transfection reagent specific for hydrogel. In this way, hydrogel-based 3D matrices activated with 3D-FectIN™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation...
- Highly efficient: cell lines and primary cells
- Specific for 3D Hydrogels
- Compatible with all types of nucleic acids
- Completely biodegradable
- Long term protein expression
- Serum Compatible
- 250µL (TN30250): Good for 65 transfections with 1µg of DNA
- 500µL (TN30500): Good for 125 transfections with 1µg of DNA
- 1000µL (TN31000): Good for 250 transfections with 1µg of DNA
Shipping Conditions: Room temperature
Perfect for all transfection applications in 3D hydrogels such as collagen, hyaluronic acid, PEG, fibrin, laminin:
- Tube and acini formation
- Neurite growth
- Tissue engineering
- Tissue regeneration
- Tumor invasion
- Neural differentiation
- Cellular polarization
- Tissue formation...
Suitable for all type of nucleic acids including: plasmid DNA, linearized DNA, double stranded RNA, mRNA, shRNA, oligonucleotides
RECOMMENDED FOR: Transfection of cells growing in 3D-hydrogels
Figure 1: Transfection of various cells on different gels with 3D-fectin.
Figure 2: Collagen-derived hydrogels were loaded with pVectOZ-SEAP complexed with 3D-FectIN™ (1μg / 3 μL) or other transfection reagents according to the manufacturers’ recommendations. 3T3 cells were seeded onto the pre-loaded matrix and alkaline phosphatase (SEAP) secretion was analysed over 4 days.