Product Description

Siglec-5/14 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4000-50, ES4000-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: SIGLEC5, CD33L2, OBBP2, Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2, CD170, SIGLEC14, Sialic acid-binding Ig-like lectin 14, Siglec-14

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from Sialic acid-binding Ig-like lectin 5/Sialic acid-binding Ig-like lectin 14 at AA range: 91-140

Isotype: IgG

Target: Siglec-5/14

Specificity: Siglec-5/14 Polyclonal Antibody detects endogenous levels of Siglec-5/14 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed by monocytic/myeloid lineage cells, Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus, Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation,

Observed Band (KD): 60kD

Function: Domain: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases
Function: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
Online Information: Siglec-5,Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain
Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains
Tissue Specificity: Expressed by monocytic/myeloid lineage cells. Found at high l

Subcellular Location: Membrane, Single-pass type I membrane protein

Storage and Stability: -20°C/1 year

Gene Name: SIGLEC5/SIGLEC14

Protein Name: Sialic acid-binding Ig-like lectin 5/Sialic acid-binding Ig-like lectin 14

Mouse SwissProt Number: Q920G3

Research Use Only