Product Description

Simian BMP5 (Bone Morphogenetic Protein 5) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11637-48, ELK11637-96, ELK11637-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: BMP 5, BMP-5, Bmp5, BMP5

Sensitivity: 5.1 pg/mL

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids.

Assay Length: 3.5h

Research Area: Signal Transduction, Tumor Immunity, Developmental Science, Bone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian BMP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian BMP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian BMP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian BMP5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only