Product Description

Simian CYFRA21-1 (CytokeSimianin Fragment Antigen 21-1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11530-48, ELK11530-96, ELK11530-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: CYFRA21-1

Sensitivity: 0.117 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Tumor Immunity, Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CYFRA21-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CYFRA21-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CYFRA21-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only