Product Description

Simian F11 (Coagulation Factor XI) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11726-48, ELK11726-96, ELK11726-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: FXI, PTA, Fletcher Factor, Prekallikrein, Plasma Thromboplastin Antecedent

Sensitivity: 1.21 ng/mL

Assay Type: Sandwich

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Plasma

Assay Length: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian F11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian F11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian F11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian F11 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only