Product Description

Simian GITR (Glucocorticoid Induced Tumor Necrosis Factor Receptor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11992-48, ELK11992-96, ELK11992-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Simian

Alternate Names: TNFRSF18, AITR, Tumor Necrosis Factor Receptor Superfamily 18, Glucocorticoid-induced TNFR-related protein, Activation-inducible TNFR family receptor

Sensitivity: 0.54 ng/mL

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Tumor Immunity, Immune Molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian GITR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian GITR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian GITR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian GITR in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only