Product Description

Simian GROa/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK11519-48, ELK11519-96, ELK11519-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CXCL1, GRO1, GROa, GRO-A, MGSA-A, NAP3, SCYB1, Chemokine C-X-C-Motif Ligand 1, Melanoma Growth Stimulating Activity Alpha, Fibroblast Secretory Protein, Neutrophil Activating Protein 3

Reactivity: Simian

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cytokine, Tumor Immunity, Infection Immunity

Assay Length: 3.5h

Sensitivity: 5.8 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only